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dsred-tagged rab7 wild-type (wt) (plasmid #12661)  (Addgene inc)


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    Structured Review

    Addgene inc dsred-tagged rab7 wild-type (wt) (plasmid #12661)
    Effect of dominant negative Rab GTPases on ABLV G-mediated viral entry. HEK293T cells expressing <t>dsRed-tagged</t> WT and DN <t>Rab5,</t> <t>Rab7,</t> and Rab11were infected with maxGFP encoding rVSV that express ABLVp G, ABLVs G, or VSV G at a MOI = 3 for 8 hrs or with rVSV that expresses EboGP at a MOI = 15 for 20 hrs and then analyzed as described in Figure . Infection of all cells was assessed. Under these conditions, the chosen MOIs yielded 40-50% virus-infected cells in controls. Results are expressed as percent virus-infected cells relative to that of WT Rab controls and represent 3 independent experiments; error bars are SEM. (A) Rab5. (B) Rab7. (C) Rab11. **, p < 0.0001; *, p < 0.005.
    Dsred Tagged Rab7 Wild Type (Wt) (Plasmid #12661), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsred-rab7+wild-type/gfp+rab7/pmc03946599-142-0-34
    Average 90 stars, based on 1 article reviews
    dsred-tagged rab7 wild-type (wt) (plasmid #12661) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Host cell virus entry mediated by Australian bat lyssavirus G envelope glycoprotein occurs through a clathrin-mediated endocytic pathway that requires actin and Rab5"

    Article Title: Host cell virus entry mediated by Australian bat lyssavirus G envelope glycoprotein occurs through a clathrin-mediated endocytic pathway that requires actin and Rab5

    Journal: Virology Journal

    doi: 10.1186/1743-422X-11-40

    Effect of dominant negative Rab GTPases on ABLV G-mediated viral entry. HEK293T cells expressing dsRed-tagged WT and DN Rab5, Rab7, and Rab11were infected with maxGFP encoding rVSV that express ABLVp G, ABLVs G, or VSV G at a MOI = 3 for 8 hrs or with rVSV that expresses EboGP at a MOI = 15 for 20 hrs and then analyzed as described in Figure . Infection of all cells was assessed. Under these conditions, the chosen MOIs yielded 40-50% virus-infected cells in controls. Results are expressed as percent virus-infected cells relative to that of WT Rab controls and represent 3 independent experiments; error bars are SEM. (A) Rab5. (B) Rab7. (C) Rab11. **, p < 0.0001; *, p < 0.005.
    Figure Legend Snippet: Effect of dominant negative Rab GTPases on ABLV G-mediated viral entry. HEK293T cells expressing dsRed-tagged WT and DN Rab5, Rab7, and Rab11were infected with maxGFP encoding rVSV that express ABLVp G, ABLVs G, or VSV G at a MOI = 3 for 8 hrs or with rVSV that expresses EboGP at a MOI = 15 for 20 hrs and then analyzed as described in Figure . Infection of all cells was assessed. Under these conditions, the chosen MOIs yielded 40-50% virus-infected cells in controls. Results are expressed as percent virus-infected cells relative to that of WT Rab controls and represent 3 independent experiments; error bars are SEM. (A) Rab5. (B) Rab7. (C) Rab11. **, p < 0.0001; *, p < 0.005.

    Techniques Used: Dominant Negative Mutation, Expressing, Infection

    Related Articles

    Plasmid Preparation:

    Article Title: Host cell virus entry mediated by Australian bat lyssavirus G envelope glycoprotein occurs through a clathrin-mediated endocytic pathway that requires actin and Rab5
    Article Snippet: A plasmid expressing eGFP (pEGFP-C1; Clonetech, Mountain View, CA) was used as an additional control in Eps15 experiments. .. DsRed-tagged Rab7 wild-type (WT) (plasmid #12661) and DN (T22N; plasmid #12662), DsRed-tagged Rab11 WT (plasmid #12679) and DN (S25N; plasmid #12680) [ ], and mRFP-tagged Rab5 WT (plasmid #14437) [ ] were purchased from Addgene, Cambridge, MA. .. RFP-tagged Rab5 DN (S34N) was generated by site-directed mutagenesis of Rab5 WT using a QuikChange II site-directed mutagenesis kit (Stratagene, Cedar Creek, TX).

    Dominant Negative Mutation:

    Article Title: Rapid and facile quantitation of polyplex endocytic trafficking.
    Article Snippet: AllStars negative control siRNA fluorescently tagged with AlexaFluor 647 (AF647-siRNA) was purchased from Qiagen (Germantown, MD). .. Plasmids encoding red fluorescent protein fusions with LAMP-1 (#181738), Rab7 (#14436) and Rab5 (#1443739), and wild-type Rab5 (#13050), dominant negative Rab5 (#13051), wild-type Rab7 (#12661), dominant negative Rab7 (#12662), wild-type Rab11 (#12679), and dominant negative Rab11 (#12680) were obtained from Addgene (Cambridge, MA) [12, 13, 31, 32]. ..



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    Effect of dominant negative Rab GTPases on ABLV G-mediated viral entry. HEK293T cells expressing dsRed-tagged WT and DN Rab5, <t>Rab7,</t> and Rab11were infected with maxGFP encoding rVSV that express ABLVp G, ABLVs G, or VSV G at a MOI = 3 for 8 hrs or with rVSV that expresses EboGP at a MOI = 15 for 20 hrs and then analyzed as described in Figure . Infection of all cells was assessed. Under these conditions, the chosen MOIs yielded 40-50% virus-infected cells in controls. Results are expressed as percent virus-infected cells relative to that of WT Rab controls and represent 3 independent experiments; error bars are SEM. (A) Rab5. (B) Rab7. (C) Rab11. **, p < 0.0001; *, p < 0.005.
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    Figure 2. Confocal fluorescence micrographs of HeLa-luc cells expressing (A) GFP-Rab5, (B) <t>GFP-Rab7</t> or (C) GFP-LAMP1 (green) and transfected with PEI/AF647-siRNA polyplexes (red) at various times post-transfection as indicated. Following deconvolution and addition of 10 z- slices of the original micrographs, colocalized pixels are shown in white in the second row of each panel. All images are 203 x 203 µm2.
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    Figure 2. Confocal fluorescence micrographs of HeLa-luc cells expressing (A) GFP-Rab5, (B) <t>GFP-Rab7</t> or (C) GFP-LAMP1 (green) and transfected with PEI/AF647-siRNA polyplexes (red) at various times post-transfection as indicated. Following deconvolution and addition of 10 z- slices of the original micrographs, colocalized pixels are shown in white in the second row of each panel. All images are 203 x 203 µm2.
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    Image Search Results


    Effect of dominant negative Rab GTPases on ABLV G-mediated viral entry. HEK293T cells expressing dsRed-tagged WT and DN Rab5, Rab7, and Rab11were infected with maxGFP encoding rVSV that express ABLVp G, ABLVs G, or VSV G at a MOI = 3 for 8 hrs or with rVSV that expresses EboGP at a MOI = 15 for 20 hrs and then analyzed as described in Figure . Infection of all cells was assessed. Under these conditions, the chosen MOIs yielded 40-50% virus-infected cells in controls. Results are expressed as percent virus-infected cells relative to that of WT Rab controls and represent 3 independent experiments; error bars are SEM. (A) Rab5. (B) Rab7. (C) Rab11. **, p < 0.0001; *, p < 0.005.

    Journal: Virology Journal

    Article Title: Host cell virus entry mediated by Australian bat lyssavirus G envelope glycoprotein occurs through a clathrin-mediated endocytic pathway that requires actin and Rab5

    doi: 10.1186/1743-422X-11-40

    Figure Lengend Snippet: Effect of dominant negative Rab GTPases on ABLV G-mediated viral entry. HEK293T cells expressing dsRed-tagged WT and DN Rab5, Rab7, and Rab11were infected with maxGFP encoding rVSV that express ABLVp G, ABLVs G, or VSV G at a MOI = 3 for 8 hrs or with rVSV that expresses EboGP at a MOI = 15 for 20 hrs and then analyzed as described in Figure . Infection of all cells was assessed. Under these conditions, the chosen MOIs yielded 40-50% virus-infected cells in controls. Results are expressed as percent virus-infected cells relative to that of WT Rab controls and represent 3 independent experiments; error bars are SEM. (A) Rab5. (B) Rab7. (C) Rab11. **, p < 0.0001; *, p < 0.005.

    Article Snippet: DsRed-tagged Rab7 wild-type (WT) (plasmid #12661) and DN (T22N; plasmid #12662), DsRed-tagged Rab11 WT (plasmid #12679) and DN (S25N; plasmid #12680) [ ], and mRFP-tagged Rab5 WT (plasmid #14437) [ ] were purchased from Addgene, Cambridge, MA.

    Techniques: Dominant Negative Mutation, Expressing, Infection

    Effect of dominant negative Rab GTPases on ABLV G-mediated viral entry. HEK293T cells expressing dsRed-tagged WT and DN Rab5, Rab7, and Rab11were infected with maxGFP encoding rVSV that express ABLVp G, ABLVs G, or VSV G at a MOI = 3 for 8 hrs or with rVSV that expresses EboGP at a MOI = 15 for 20 hrs and then analyzed as described in Figure . Infection of all cells was assessed. Under these conditions, the chosen MOIs yielded 40-50% virus-infected cells in controls. Results are expressed as percent virus-infected cells relative to that of WT Rab controls and represent 3 independent experiments; error bars are SEM. (A) Rab5. (B) Rab7. (C) Rab11. **, p < 0.0001; *, p < 0.005.

    Journal: Virology Journal

    Article Title: Host cell virus entry mediated by Australian bat lyssavirus G envelope glycoprotein occurs through a clathrin-mediated endocytic pathway that requires actin and Rab5

    doi: 10.1186/1743-422X-11-40

    Figure Lengend Snippet: Effect of dominant negative Rab GTPases on ABLV G-mediated viral entry. HEK293T cells expressing dsRed-tagged WT and DN Rab5, Rab7, and Rab11were infected with maxGFP encoding rVSV that express ABLVp G, ABLVs G, or VSV G at a MOI = 3 for 8 hrs or with rVSV that expresses EboGP at a MOI = 15 for 20 hrs and then analyzed as described in Figure . Infection of all cells was assessed. Under these conditions, the chosen MOIs yielded 40-50% virus-infected cells in controls. Results are expressed as percent virus-infected cells relative to that of WT Rab controls and represent 3 independent experiments; error bars are SEM. (A) Rab5. (B) Rab7. (C) Rab11. **, p < 0.0001; *, p < 0.005.

    Article Snippet: DsRed-tagged Rab7 wild-type (WT) (plasmid #12661) and DN (T22N; plasmid #12662), DsRed-tagged Rab11 WT (plasmid #12679) and DN (S25N; plasmid #12680) [ ], and mRFP-tagged Rab5 WT (plasmid #14437) [ ] were purchased from Addgene, Cambridge, MA.

    Techniques: Dominant Negative Mutation, Expressing, Infection

    Figure 2. Confocal fluorescence micrographs of HeLa-luc cells expressing (A) GFP-Rab5, (B) GFP-Rab7 or (C) GFP-LAMP1 (green) and transfected with PEI/AF647-siRNA polyplexes (red) at various times post-transfection as indicated. Following deconvolution and addition of 10 z- slices of the original micrographs, colocalized pixels are shown in white in the second row of each panel. All images are 203 x 203 µm2.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Rapid and facile quantitation of polyplex endocytic trafficking.

    doi: 10.1016/j.jconrel.2016.12.035

    Figure Lengend Snippet: Figure 2. Confocal fluorescence micrographs of HeLa-luc cells expressing (A) GFP-Rab5, (B) GFP-Rab7 or (C) GFP-LAMP1 (green) and transfected with PEI/AF647-siRNA polyplexes (red) at various times post-transfection as indicated. Following deconvolution and addition of 10 z- slices of the original micrographs, colocalized pixels are shown in white in the second row of each panel. All images are 203 x 203 µm2.

    Article Snippet: Plasmids encoding red fluorescent protein fusions with LAMP-1 (#181738), Rab7 (#14436) and Rab5 (#1443739), and wild-type Rab5 (#13050), dominant negative Rab5 (#13051), wild-type Rab7 (#12661), dominant negative Rab7 (#12662), wild-type Rab11 (#12679), and dominant negative Rab11 (#12680) were obtained from Addgene (Cambridge, MA) [12, 13, 31, 32].

    Techniques: Fluorescence, Expressing, Transfection

    Figure 3. Colocalization (determined by Mander’s coefficient) of AF647-siRNA with (A) GFP- Rab5, (B) GFP-Rab7 and (C) GFP-LAMP1 in HeLa-luc cells as a function of time post- transfection with PEI/AF647-siRNA polyplexes. Colocalization was determined in eight cells from three separate micrographs. (error bars represent standard deviation.)

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Rapid and facile quantitation of polyplex endocytic trafficking.

    doi: 10.1016/j.jconrel.2016.12.035

    Figure Lengend Snippet: Figure 3. Colocalization (determined by Mander’s coefficient) of AF647-siRNA with (A) GFP- Rab5, (B) GFP-Rab7 and (C) GFP-LAMP1 in HeLa-luc cells as a function of time post- transfection with PEI/AF647-siRNA polyplexes. Colocalization was determined in eight cells from three separate micrographs. (error bars represent standard deviation.)

    Article Snippet: Plasmids encoding red fluorescent protein fusions with LAMP-1 (#181738), Rab7 (#14436) and Rab5 (#1443739), and wild-type Rab5 (#13050), dominant negative Rab5 (#13051), wild-type Rab7 (#12661), dominant negative Rab7 (#12662), wild-type Rab11 (#12679), and dominant negative Rab11 (#12680) were obtained from Addgene (Cambridge, MA) [12, 13, 31, 32].

    Techniques: Transfection, Standard Deviation